Primary Responsibilities Summary
Postdoctoral Scholar
- Responsible for writing and reviewing scientific manuscripts for publications
- Grant writing (K99/R00, NIH Loan Repayment)
- Manage collaborations between multiple laboratories
- Laboratory Safety Officer: Responsible for clinical and laboratory compliance with local, state and federal laws and regulations
- Techniques used
- Bacterial Metabolomics
- Comparative genomics
- Bacterial gene mutagenesis
- Bacterial Biofilms (biomass, glycogen, pH, glucan production, environmental stress)
- DNA/RNA sequencing including RT-PCR, RNA Sequencing and 16S sequencing
- Animal models with Drosophila and rat caries models
- Data analysis with Excel, CLC DNA Workbench Software, antiSMASH, Galaxy, XCMS, MSDial, Metaboanalyst, Snapgene
DART Postdoctoral Scholar
- Responsible for writing and reviewing scientific manuscripts for publications
- Grant writing (F32, Diversity Supplement, K99/R00, NIH Loan Repayment, L'oreal WIS)
- Supervise and train dental residents, graduate, undergraduate and high school students for multiple research projects
- Maintain storage system for clinical isolates
- Manage collaborations between multiple laboratories
- Laboratory Safety Officer: Responsible for clinical and laboratory compliance with local, state and federal laws and regulations
- Techniques used
- Whole Genome Sequencing (WGS) and comparative genomics
- Bacterial gene mutagenesis
- Microbiome Analysis
- Bacterial Biofilms (biomass, glycogen, pH, glucan production, environmental stress)
- DNA/RNA sequencing including RT-PCR, RNA Sequencing
- Data analysis with Excel, CLC DNA Workbench Software, antiSMASH, Galaxy, Snapgene
DART Pre-doctoral Fellow
- Responsible for writing and reviewing scientific manuscripts for publications
- Grant writing (F31 and NSF)
- Supervise and train dental residents, graduate and undergraduate students for multiple research projects
- Maintain storage system for clinical isolates
- Streamline and problem solving for operations between multiple laboratories
- Laboratory Safety Officer: Responsible for clinical and laboratory compliance with local, state and federal laws and regulations
- Techniques used
- RT-PCR using Bio-Rad IQ5 with SYBR Green or Plexor (multiplex) systems
- Data analysis with Plexor Analysis Software, Excel, CLC DNA Workbench Software, SPSS
- Multi Locus Sequence Typing
- DiversiLab rep-PCR web-based analysis tools
Research Associate/Research Assistant
- Isolate, store, and process clinical isolates of Streptococcus mutans for RO1 Grant Study (currently 32,000 isolates)
- Responsible for writing and reviewing scientific manuscripts for publications
- Supervise and train dental residents, graduate and undergraduate students for multiple research projects
- Develop and maintain storage system for clinical isolates
- Streamline and problem solving for operations between multiple laboratories
- Prepare budget cost benefit analysis and product quality assessments for laboratory purchasing
- Laboratory Safety Officer: Responsible for clinical and laboratory compliance with local, state and federal laws and regulations
- Techniques used
- RT-PCR using Bio-Rad IQ5 with SYBR Green or Plexor (multiplex) systems
- Data analysis with Plexor Analysis Software, Excel, CLC DNA Workbench Software
- Multi Locus Sequence Typing (MLST)
- DiversiLab rep-PCR web-based analysis tools
Quality Assurance Officer
- Responsible for Laboratory Management including all safety training, annual safety audits preparation, equipment maintenance, supply inventory, and waste management
- Perform research and development of methods for monitoring heterotrophic bacterial counts in dental unit waterlines
- Manage the Compliance Databases for School of Dentistry including training, vaccinations, and monitoring
- Assist Director of Clinical Operations with patient satisfaction, risk management assessments, and legal issues
- Investigate, develop and implement clinic policies and procedures
- Laboratory Safety Officer: Responsible for clinical and laboratory compliance with local, state and federal laws and regulations
Program Coordinator II
- Perform research and development of new testing protocols for environmental Legionella testing for University Hospital
- Monitor and perform routine biological monitoring of autoclaves with Geobacillus stearothermophilus and Bacillus atrophaeus for School of Dentistry, medical and dental practices.
- Manage the business operations for Service Center including financial responsibilities, order processing, marketing, and customer service.
- Budget Analysis and annual report preparation.
- Laboratory Safety Officer: Responsible for clinical and laboratory compliance with local, state and federal laws and regulations
Research Assistant
- Technical position performing basic microbiological culture techniques
- Responsible for multiple multidisciplinary PCR and electrophoresis projects.
- Coordinate research efforts between multiple projects.
- Media preparation and laboratory maintenance.
- Techniques used:
- Denaturing gradient & temperature gradient gel electrophoresis
- Arbitrarily Primed PCR, agarose gel electrophoresis
- Chromosomal DNA fingerprinting, Enzyme digests
- DNA Extraction and Purification
- Gene cloning
- Bacterial culture methods and identification (morphological, biochemical, and Gram staining)
- Spectrophotometry
- pH monitoring
- Media preparation
Medical Technologist in Training
- Perform routine clinical testing
- Routine maintenance and repairs on equipment
- Techniques used:
- Immunoelectrophoresis (protein gel electrophoresis) on serum, spinal fluid, urine, and body fluids
- Isoenzyme fractionation electrophoresis for cardiac enzymes creatine phosphokinase (CPK) and lactate dehydrogenanse (LDH)
- Thin layer liquid chromatography for fetal pulmonary maturity
- High-pressure liquid chromatography for glycohemoglobin (GHb A1c)
- Lipoprotein electrophoresis for cholesterol lipid profiles for high and low density lipoprotein cholesterols (HDL-C, LDL-C) and triglycerides
- Sickle Cell solubility test
Laboratory Assistant
- Accessioning of clinical samples
- Delivery and shipment of clinical samples
- Laboratory Safety Training (e. g. Dry ice shipping, blood borne-pathogens,etc.)